Analysis of whole blood by ICP-MS equipped with a high temperature total sample consumption system

Autores

Canabate, A; Garcia-Ruiz, ME; Resano, M; Todoli, J

Revista

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY

Año: 2017 Volumen: 32 (1) Páginas: 78-87

Editor:

ROYAL SOC CHEMISTRY

DOI:

10.1039/c6ja00374e

Resumen

In this work, the performance of a high temperature torch Integrated Sample Introduction System (hTISIS) using inductively coupled plasma mass spectrometry (ICP-MS) for the multi-element analysis of whole blood is evaluated. Two different quadrupole-based spectrometers were tested and the optimization studies were performed with a reference whole blood material. Sample dilution factor and hTISIS temperature were taken as the variables. The sample was introduced following two different procedures: continuous aspiration and air-segmentation. The optimum performance of the system in terms of both analytical figures of merit and accuracy was obtained for a hTISIS temperature of 200 degrees C and for either 1 : 10 or 1 : 25 dilution factors depending on the ICP-MS device used. An improvement in terms of sensitivity and detection limits was obtained with the hTISIS as compared to the conventional cyclonic spray chamber. Thus, for 1 : 25 diluted blood samples, the hTISIS improved the sensitivity by a factor ranging 2.0-4.5. Moreover, at 200 degrees C, the limits of detection for hTISIS at 200 degrees C were 1.1 to 8.4 times lower than those for the cyclonic spray chamber. This analytical parameter was in the ng L-1 range for the detected elements. Matrix effects in turn became less severe as the hTISIS temperature increased (from room temperature to 80 degrees C). All these results were obtained without a severe degradation of the fundamental plasma parameters. In fact, under optimum conditions BaO+/Ba+ and Ba2+/Ba+ signal ratios were 1.2\% and 2.0\%, respectively. The developed method was applied for the analysis of low volumes (ca. 2.5 mu L) of real blood samples after a minimally invasive collection by volumetric absorptive microsampling. The hTISIS is an easy-to-implement sample introduction system that can be employed for the routine analysis using ICP-MS.

Afiliación

Todoli, JL (Reprint Author), Univ Alicante, Dept Analyt Chem Nutr & Food Sci, POB 99, E-03080 Alicante, Spain.
Canabate, Agueda; Todoli, Jose-Luis, Univ Alicante, Dept Analyt Chem Nutr & Food Sci, POB 99, E-03080 Alicante, Spain.
Garcia-Ruiz, Esperanza; Resano, Martin, Univ Zaragoza, Dept Analyt Chem, Aragon Inst Engn Res I3A, Pedro Cerbuna 12, E-50009 Zaragoza, Spain.