Contact:
- Nieves Movilla: tecnicomicros.i3a@unizar.es
Confocal LightSheet microscope
Leica STELLARIS 5 DLS
Confocal microscopy enables high-resolution imaging by eliminating out-of-focus light via a pinhole, producing sharp 3D reconstructions of cells, tissues and fluorescent samples. The Digital LightSheet (DLS) module adds light-sheet illumination, which is ideal for thick samples, reducing phototoxicity and enabling rapid volumetric acquisitions.
Techniques and capabilities:
- Fluorescence microscopy
- Confocal reflection
- Time-lapse with incubation (temperature, humidity, CO₂ / O₂ – normoxia)
- 3D reconstruction
- Digital lightsheet (DLS) for organoids, tissues and 3D cultures
Compatible samples:
Cells, tissues, organoids, embryos, fixed samples and materials with a reflective surface.
More information:
Multiphoton microscope
Leica STELLARIS 8 DIVE
Confocal microscopy enables high-resolution imaging by eliminating out-of-focus light via a pinhole, producing sharp 3D reconstructions of cells, tissues and fluorescent samples. The Digital LightSheet (DLS) module adds light-sheet illumination, which is ideal for thick samples, reducing phototoxicity and enabling rapid volumetric acquisitions.
Techniques and capabilities:
- Second Harmonic Signal (SHG)
- Fluorescence lifetime measurement (FLIM) studies
- In-depth reconstruction
- Advanced analysis with Aivia (AI for segmentation and quantification)
Compatible samples:
Live or fixed tissues, organoids, 3D cultures, collagen (SHG), samples with deep-tissue fluorophores.
More information:
MICA WideFocal Microscope
Leica
The Leica MICA WideFocal system integrates transmitted light and fluorescence illumination with four light sources (405, 488, 561 and 638 nm) and enables the simultaneous acquisition of up to 4 fluorochromes via four 5 MP monochrome cameras with real-time spectral separation. It features 1.6x, 10x, 20x and 63x (water immersion) objectives, a motorised XY stage compatible with multi-well plates (384-well to 6-well), Petri dishes and slides. It includes multidimensional software for mosaics and multiple positions, automatic fluorescence optimisation and the THUNDER image enhancement method.
Techniques and capabilities:
- Optical microscopy
- Wide-field and confocal fluorescence microscopy
- High-throughput applications on multi-well plates
More information:
https://www.leica-microsystems.com/products/p/mica/specification/
AIVIA software
AI Image Analysis
Aivia is an advanced image analysis software programme based on artificial intelligence, designed to process, segment and quantify data from any microscope. It includes a self-learning pixel classifier, an extensive collection of analysis recipes and automatic updates to the latest available version, ensuring accurate and reproducible results.
Technical specifications:
- AI-based image analysis software
- Pixel classifier with self-learning technology
- Wide range of analysis presets
- Automatic updates to the latest version
Applications:
- Analysis of images captured using any microscope
More information:
https://www.leica-microsystems.com/es/productos/software-de-microscopia/p/aivia/
Funding
The equipment at the Advanced Microscopy Service of the Aragón Institute for Engineering Research (I3A), University of Zaragoza, forms part of project EQC2021-007464-P, funded by MCIN/AEI/10.13039/501100011033 and by the European Union’s “NextGenerationEU” programme/ PRTR.
This initiative forms part of the 2021 Call for Proposals for the Procurement of Scientific and Technical Equipment (EQC2021), funded by the Ministry of Science and Innovation, the State Research Agency and the European Union through the Recovery, Transformation and Resilience Plan (PRTR).